Salusin-Î± and salusin-Î² are recently discovered bioactive endogenous peptides with both haemodynamic and mitogenic activity. Salusin-Î± and salusin-Î² immunoreactivity has been detected in the stomach and intestine. Our study is designed to examine the oxidative stress, cytokines and histological effects of administration of salusin-Î± and salusin-Î² on cold-restricted stress (CRS)-induced gastric ulcers. A total of 32 Sprague Dawley, male rats were divided into four groups randomly. Group1: Control; Group2: CRS (Rats were placed individually in the restriction chamber and were subjected to the cold restricted stress at 4°C for 4 h); Group3: CRS+5nmol/kg Salusin-Î±; Group4: CRS+5nmol/kg Salusin-Î². We determined malondialdehyde (MDA), myeloperoxidase (MPO), superoxide dismutase (SOD), salusin-Î± and salusin-Î² levels from stomach tissue, tumor necrosis factor-Î± (TNF-Î±) and interleukin-1Î² (IL-1Î²)) levels from serum. Multiple comparison tests of Kruskal-Wallis and Dunn have been used for the analysis of the data. All the results were presented as mean±SD. When compared to the control group; while salusin-Î± level significantly increases in the group to which CRS has been applied, Salusin-Î² has shown a slight increase. While MDA, MPO, TNF-Î±, IL1-1Î², and SOD activity in group subjected to the CRS changed, in the groups administered salusin-Î± and salusin-Î², MDA, MPO and TNF-Î± levels decreased, and SOD activity and IL-1Î² levels increased. The mucosal injury and caspase-3 expression increasing with the application of CRS in the histological examinations decreased with the application of salusins. The suppression of salusin-Î± and salusin-Î² on caspase-3 expression by means of their effects on oxidative injury and TNF-Î± levels shows that these two hormones could be an anti-ulcerative agent.
Ulcer, Cold-restricted stress, Salusin, Cytokines, Oxidative stress