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Original Article

Polymorphism of 8 X-Chromosomal STR loci in Turkish population

Erhan Acar, Gonul Filoglu, Ozlem Bulbul

   

Abstract
In forensic sciences, X-chromosomal short tandem repeats (X-STRs), a complementary marker to the other genetic markers (autosomal, Y-chromosomal or mitochondrial), can be used in complex kinship analysis. Especially when a biological sample is not available from the putative father and samples from paternal relatives could be used instead. In this study eight X-chromosomal STR loci (DXS7132, DXS7423, DXS8378, DXS10074, DXS10101, DXS10134, DXS10135, HPRTB and Amelogenin) were investigated in population samples of 129 unrelated healthy Turkish volunteer [male (n=56), female (n=73)]. X-STRs were amplified in a multiplex PCR reaction with Mentype® Argus X-8 Kit. The separation and detection of PCR products were performed by capillary electrophoresis on a 310 Genetic Analyzer (Applied Biosystems). Statistical analysis was performed using POPgene v.1.32. and Arlequin v3.1 software’s. Data was compared with Turkish populations living in Europe (Germany and Denmark) and other populations (Japan, Ghana, Finland, Portugal and Germany). The high genetic distances were observed for the geographically distant populations while no significant differences found between in studied and tested Turkish populations. Results showed that eight STR loci on the X chromosome in Turkish population were found as highly polymorphic and useful for forensic applications.

Key words: X-STR, kinship analysis, Turkish population

Med-Science. 2018; 7(3): 672-6

 
Medicine Science Vol:7 Issue:3 Year:2018 PP:469-723
Posted in Vol: 7 Issue: 3 Year: 2018 September pp: 469-723

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