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Investigation of prognostic significance of CD44 expression in women with vulvar squamous cell carcinoma

Original Article

Investigation of prognostic significance of CD44 expression in women with vulvar squamous cell carcinoma

Yakup Yalcin, Serenat Eris Yalcin, Selda Uysal, Burak Tatar, And Yavuz, Mehmet Ozgur Akkurt, Seyran Yigit

   

Abstract
The aim of our study is to evaluate whether CD 44 isoform expression is a prognostic factor in vulvar carcinom and to correlate the expression with clinicopathological parameters. Methods: The study included 26 patients diagnosed with invasive squamous cell vulvar cancer. The data of patients were obtained from the oncology follow-up records and the hospital database. The haemotoxylene & eosin stained preparates of the cases were removed from the archives and re-evaluated by examination of the slides under a light microscope. The preparates of the patients were evaluated separately according to the extent and intensity of staining and the combined score applied with the immunohistochemical method in the anti-CD44 antibody in the epithelium and the stroma. Results: When the CD44 epithelial staining intensity and staining combined scores of the patients were examined according to other clinicopathological parameters, a statistically significant difference was found between the groups in terms of tumour grade, differentiation, age, lymph node positivity, and survival rates (p

Key words: CD44, expression, vulva, vulvar carcinom

Med-Science. 2019; 8(4): 774-781

 
 
 
Medicine Science Vol:9 Issue:1 Year:2020 PP:1–288

The value of the neutrophil to lymphocyte ratio, platelet to lymphocyte ratio and ca-125 in differential diagnosis of ovarian endometriosis

Original Article

The value of the neutrophil to lymphocyte ratio, platelet to lymphocyte ratio and ca-125 in differential diagnosis of ovarian endometriosis

Mustafa Tas

   

Abstract
The definitive diagnosis of the endometriosis relies on direct visualization at the surgery and the histopathological verification of the endometrial glands and stroma in biopsies of suspected lesions. Non-invasive tests to diagnose the endometrioma are required to avoid the complications of the laparoscopy. The present study was carried out to compare the neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, and CA-125 level in patients with endometrioma and in women with other benign ovarian cysts. Retrospective medical data of 73 women who underwent open or laparoscopic surgery for ovarian cysts and 40 healthy controls were analyzed. Patients with endometriomas, other benign ovarian cysts and controls were compared with respect to age, CBC parameters, and Ca-125 measurements to identify the factors associated with the presence of the endometrium. There were no significant differences among the three groups with respect to leukocyte count, neutrophil, lymphocyte, plateletcrit, reticulocyte distribution width, hemoglobin level, platelet count, and mean platelet volume. The neutrophil to lymphocyte ratio and platelet to lymphocyte ratio were also similar in all groups. However, Ca-125 level was significantly higher in patients with endometrioma [86 (36-130) U/ml] compared to that of the benign ovarian cyst group [21.5 (6-62) U/ml] and the healthy controls [26.5 (9-51) U/ml]. ROC curve analysis provided that a cut-off value of 44.5 U/ml could predict the presence of endometrioma with a sensitivity of 97 % and with a specificity of 96 %. Our findings clearly demonstrate that neither neutrophil to lymphocyte ratio nor platelet to lymphocyte ratio has any value in distinguishing women with endometrioma from those with other benign ovarian cysts or healthy controls. However, Ca-125 levels might be used to identify patients with endometrioma, with high sensitivity and specificity.

Key words: Endometrioma, ovarian cyst, Ca-125, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio

Med-Science. 2019; 8(4): 782-5

 

Does anti-Helicobacter pylori immunohistochemical staining has a confirmative role in the definitive diagnosis of negative gastric biopsy samples stained with routine histological stains?

Original Article

Does anti-Helicobacter pylori immunohistochemical staining has a confirmative role in the definitive diagnosis of negative gastric biopsy samples stained with routine histological stains?

Cengiz Kocak, Sirin Kucuk, Ersoy Ercihan, Mehmet Gundogan, Canan Sakar, Asli Ucar Uncu, Bulent Mizrak

   

Abstract
Helicobacter pylori (H. pylori) causes chronic gastritis, and is associated with gastric lymphoma and carcinoma. Although severe infections with many bacteria can be identified easily on hematoxylin-eosin (H&E)–stained tissue samples; the identification ratio is only 66% with many false-positive and false-negative results. Furthermore, identification of the low-density bacteria, primarily following the therapy, requires special staining methods. The purpose of this study was to investigate whether the use of anti-helicobacter immunohistochemical (IHC) methods in the histopathological diagnosis of H. pylori is necessary to obtain accurate results. We investigated whether the use of specific immunohistochemical methods can detect H. pylori, which previously undetected by classical histological staining methods, in endoscopic gastric biopsy specimens. The study involved 210 endoscopic gastric biopsies considered as H. pylori negative with routine histological stainings and subsequently applied IHC staining methods to confirmative diagnosis. Inflammation was seen in all cases. One hundred thirty-eight patients had chronic inactive, 72 patients had chronic active gastritis. While H. pylori was found as negative in all cases with H&E and Giemsa stainings, in 60 of all these cases, H. pylori was found as positive with anti-H. pylori IHC staining. We suggest that in cases of chronic gastritis suggestive of H. pylori infection, when H. pylori cannot be seen by routine histological stainings, the IHC staining method is a definitive diagnostic tool for identification of H. pylori to avoid from false negative results.

Key words: Gastritis, helicobacter pylori, immunohistochemistry

Med-Science. 2019; 8(4): 786-91

 
 
 

Does bone mineral density change in polycystic ovarian syndrome?

Original Article

Does bone mineral density change in polycystic ovarian syndrome?

Funda Dinc Elibol, Sezen Bozkurt Koseoglu

   

Abstract
Polycystic ovarian syndrome (PCOS), is the most frequent endocrine disorder in reproductive age and PCOS has effects on bone metabolism. There is conflicting data on the effect of PCOS on bone metabolism. The aim of this study to evaluate whether bone mineral density (BMD) changes in PCOS or not. Twenty-seven patients with PCOS for the study group and 27 patients for the control group were included in this retrospective study. BMD measurements with abdomen CTs were taken for each lumbar vertebra (from vertebra L1 to vertebra L5) and sacral 1st vertebra. BMD measurements were recorded as HU. The lumbar 5th vertebra’s BMD was 238.70±47.80 HU in the PCOS group and 264.91±36.62 in the control group and the difference was statistically significant (P=0.033). BMD values of all vertebras were lower in the PCOS group than the control group, but statistically, significance was seen in only the lumbar 5th vertebra. BMD values were found to be lower in PCOS than the control group for all vertebras in this study. Even though there is no scanning method recommend to women in PCOS for BMD according to the current guidelines and literature, we think these patients should be evaluated more carefully in advanced ages in the light of the results of our study.

Key words: Bone mineral density, CT, DEXA, PCOS, vertebra

Med-Science. 2019; 8(4): 792-5

 

Anatomical and radiologic approach to tracheal diverticula

Original Article

Anatomical and radiologic approach to tracheal diverticula

Selma Caliskan, Emre Can Celebioglu, Sinem Akkasoglu, Ibrahim Tanzer Sancak

   

Abstract
Tracheal diverticula (TD) are benign conditions characterized by single or multiple outpouchings from the tracheal wall. Different series which demonstrate tracheal diverticula on autopsy series, fiber optic bronchoscope examinations, and CT images are found in the literature. Treatment options for TD are surgical resection, endoscopic cauterization with laser or electrocoagulation and conservative management. Surgery is the treatment choice for young patients, while conservative treatment is recommended for elderly patients. We performed a retrospective study on 2271 patients who underwent thoracic CT examination for different reasons. Presence, number, anatomical location, level, diameter, cystic component, and accompanying variations were assessed and recorded. A total of 2271 patients were evaluated in the present study. 73 patients (38 females, 35 males) with TD were detected. The prevalence of TD was found to be 3.2%. It was found more frequently in women (52.1%) than in men (47.9%). The mean diameter of the diverticula was 4.5 mm (range 1-15 mm) in females and 5mm (1-14) in males. There was no difference between gender groups according diameter median values (p=0.811). TD was solitary in 71 (97.2%) patients and doubled (2.7%) in two patients. In conclusion our data showed a female predilection of prevalence, but no statistically significant difference between gender and other parameters (diameter, side, and level) was indicated. Patients with paratracheal air cysts that have no connection with the tracheal lumen were also analyzed in this study, and cystic group’s diameter values were found to be higher than the non-cystic group. This difference was found to be statistically significant.

Key words: Tracheal diverticula, computed tomography, air cyst, variation

Med-Science. 2019; 8(4): 796-9

 

Elevated blood basophil count may has a role in etiopathogenesis of isolated coronary artery ectasia

Original Article

Elevated blood basophil count may has a role in etiopathogenesis of isolated coronary artery ectasia

Mucahid Yilmaz, Hidayet Kayancicek, Nevzat Gozel, Yusuf Cekici, Mehmet Nail Bilen, Guney Sarioglu, Fikret Keles, Hasan Korkmaz

   

Abstract
The pathophysiology of isolated coronary artery ectasia (CAE) takes in inflammation and atherosclerosis. It is clear that basophils have a critical role in endothelial dysfunction, atherosclerosis and inflammation. In this study, it was aimed to examine the association between isolated CAE and basophilia. All cases who underwent coronary angiography between January 2013 and April 2018 evaluated retrospectively. Of 10985 cases, 173 (107 males) with isolated CAE and 220 with normal coronary angiography (NCA) that age and gender matched subjects (119 males) were recorded. Hospital’s database was used to derive the biochemical and hematological test results, and baseline characteristics. White blood cell (WBC) count and basophil count were significantly elevated for the cases that have angiographic isolated CAE when compared to the subjects with NCA [ 7.87 (6.83-9.42)109/ L vs 7.48 (6.27-8.67) 109/ L, p=0.01; 0.04 (0.03-0.05) 109/ L vs 0.03 (0.02-0.05) 109/ L, p=0.03, respectively]. According to receiver operating characteristics curve analyses (ROC); the specificity of a basophil value > 0.037 109/ L (measured prior to coronary angiography) in predicting isolated CAE was 57,3% and the sensitivity was 57.2% (area under the curve [AUC] 0.562, 95% CI 0.505, 0.618; p=0.03). Patients with isolated CAE have higher blood basophil count. Elevated blood basophil count may have a substantial mission in the pathogenesis of isolated CAE.

Key words: Isolated coronary artery ectasia, blood basophil levels, inflammation

Med-Science. 2019; 8(4): 800-6

 
 
 

Detectability of microscopic findings of fracture healing in the early stages of the healing process at various postmortem intervals and evaluation of wound vitality

Original Article

Detectability of microscopic findings of fracture healing in the early stages of the healing process at various postmortem intervals and evaluation of wound vitality

Selcuk Cetin, Dilek Durak, Ulviye Yalcinkaya, Elif Cetin, Filiz Eren, Bulent Eren, Vahide Aslihan Durak

   

Abstract
In our study, it is aimed how long do the microscopic findings of fracture healing in postmortem interval can be detected and whether the detected findings can be used in differentiation of fractures occur in the period of antemortem or postmortem or not.
In our study, microscopic findings of 10 study groups included manually fractured bone samples that were created in antemortem period in New Zealand White rabbit fibulas. The specimens, in a closed environment under constant temperature and humidity that exposed to 15-day process of putrefaction, microscopic signs of inflammation, fibrin, granulation tissue and periosteal new bone formation in fracture edges were evaluated.
In the control group, none of the evaluated parameters were detected microscopically. In microscopic evaluation of the study groups; fibrin was detected in the all study groups including postmortem 360th hour samples. As a result in our study; in bone fractures, inflammation findings, fibrin, granulation tissue and the presence of periosteal new bone formation, have indicated even in cases where advanced processes of autolysis and putrefaction or soft tissue has disappeared, it revealed significant findings in terms of vitality by histopathological examination.

Key words: Postmortem interval, forensic pathology, bone; fractures, microscopy

Med-Science. 2019; 8(4): 807-13

 
 
 

Antioxidant effect of grape molasses in rat heart tissues

Original Article

Antioxidant effect of grape molasses in rat heart tissues

Tugba Raika Kiran, Onder Otlu, Ercan Karabulut, Ahu Pakdemirli, Nermin Ozcan

   

Abstract
Grape molasses, which is rich in mineral substances such as flavonoids, polyphenol, antioxidants, iron, calcium, phosphorus, potassium, and magnesium is a natural nutrient, that is concentrated with boiling without adding any additive. In this study, we aimed to investigate the effects of molasses and resveratrol diets on oxidative stress parameters in cardiac damage induced with 7,12-dimethylbenz(a)anthracene (DMBA). A total of 42 Wistar albino female rats were divided into six groups with seven in each. Control group was administered subcutaneous injection of 1 mL mixture including 20 mL sesame oil + 30 mL Dimethyl Sulphoxyde (DMSO); DMBA group received subcutaneous injection of 10 mg/Kg DMBA on the 0th and 7th days. DMBA + Molasses group received DMBA application and feed containing 20 % molasses; DMBA + Resveratrol group received subcutaneous injection of DMBA + 10 mg/Kg resveratrol a day. Molasses group received feed with 20 % molasses, and Resveratrol group received subcutaneous injection of 10 mg/Kg resveratrol a day. There was a statistically significant difference between the DMBA group and DMBA + Molasses, control, resveratrol and molasses groups in terms of nitric oxide activity. There was a statistically significant difference between the DMBA group and DMBA + Resveratrol, DMBA + Molasses, control, molasses and resveratrol groups in term of malondialdehyde (MDA) activity. There was a statistically significant difference between DMBA groups, and DMBA + Molasses, DMBA + Resveratrol, and control groups in terms of glutathione (GSH) activity. Based on these results, it could be said that grape molasses could provide protection against oxidative stress as resveratrol, decreasing the risk of damage by free radicals.

Key words: Grape molasses, resveratrol, oxidative stress, DMBA, heart

Med-Science. 2019; 8(4): 814-9

 

The relationship of the smoking status of students in the school of health and vocational school of health services with the dependent personality trait

Original Article

The relationship of the smoking status of students in the school of health and vocational school of health services with the dependent personality trait

Fatma Soylemez, Metin Fikret Genc

   

Abstract
The purpose of this study is to investigate the relationship between the smoking prevalence and dependent personality trait of the students receiving health education in Bitlis Eren University. The students who were enrolled in and were continuing to attend Bitlis Eren University School of Health and Vocational School of Health Services in the 2011-2012 academic year were selected in the sample. The questionnaires were applied to the students under supervision between December 2011 and January 2012. The mean age of 413 students participating in the study was 20.8, and 49.9% of them were male, and 50.1% were female. While the smoking rate was 29.8% in the sample group, this rate was 45.6% in the male students and 14% in the female students. When the smokers and those who quit smoking were assigned to a group and compared with those who never smoked, the dependent personality scores of the students who were smoking or quit smoking were lower than those who never smoked before (p

Key words: Bitlis Eren University, smoking, dependent personality, university students

Med-Science. 2019; 8(4): 820-6

 
 
 

Possibility of reinnervation and prevention of distal target organ atrophy following side to side neurorrhaphy to the intact nerve after end to end repair of proximal transected peripheral nerves

Original Article

Possibility of reinnervation and prevention of distal target organ atrophy following side to side neurorrhaphy to the intact nerve after end to end repair of proximal transected peripheral nerves

Mustafa Karakaplan, Emre Ergen, Irfan Ayan, Kadir Ertem, Arslan Bora

   

Abstract
The aim of the study is to investigate the possibility of reinnervation and prevention of muscle atrophy following side to side neurorrhaphy to the intact nerve after end to end repair of proximal transected peripheral nerves in order to prevent distal target organ atrophy. For this, four groups each containing five Sprague–Dawley female rats were used. In group I, no surgical procedure was performed as a control group. In group II, side to side distal neurorrhaphy performed to the tibial and peroneal nerves after end to end repair of transected proximal tibial nerve. In group III. distally side to side epineural neurorrhaphy performed to the tibial and peroneal nerves. In group IV, end to end epineural repair was performed after proximal tibial nerve transection. The rats were followed up for 3 months for nerve regeneration. Subsequently group II, III and IV were evaluated histopathologically. In all group, tibial and fibular bony weights, foot weights, anterior and posterior crural muscle weights and EMG parameters were evaluated. Comparison between the groups revealed no significant differences regarding EMG and muscle weights between groups 2 and 4 also axonal degeneration was observed in 3 group after neurorrhaphy. As result of experimental study, we think that side to side repair of intact distal nerves as an adjunct to end to end repair of proximal nerve transections has no additional benefit to prevent distal organ atrophy but rather may be caused harm on the intact nerve. In addition, it has been observed that this technique affects the intact nerve rather than the transected nerve.

Key words: Proximal nerve transection, end to end, side to side, nerve repair

Med-Science. 2019; 8(4): 827-31