Glutathione peroxidase-lowering effects of the mangosteen pericarp extract on CCl4-induced hepatic injury in rats

Abstract
This study aimed to investigate whether an ethanolic extract of Garcinia mangostana pericarp is able to modulate the glutathione peroxidase and glutathione system in the liver of CCl4-induced hepatic injury. Thirty one male Sprague Dawley rats were divided into five groups, including control (untreated) group; CCl4 group (at dose 0.55 mg/kg body weight dissolved in coconut oil, at day eight); EEGM group (at doses 900; 1080; and 1296 mg/kg body weight/day for eight days then received CCl4 at dose 0.55 mg/kg body weight dissolved in coconut oil). Glutathione peroxidase and GSH/GSSH ratio were assayed by colorimetric method. The level of GPx was insignificantly lower in the CCl4 group compared to the untreated control group (P > 0.05). Out of the 900 mg/kg BW, 1080 mg/kg BW, and 1296 mg/kg BW doses of Garcinia mangostana extract, only the two highest doses significantly decreased in GPx level compared to the CCl4 group or untreated control group (P < 0.05). The level of the GSH/GSSG ratio in the liver homogenate were not significantly different between groups (P > 0.05). Mangosteen pericarp extract decrease the activity of glutathione peroxidase liver on CCl4-induced hepatic injury of rats.